Mouse Spermatozoa Contain a Nuclease that Is Activated by Pretreatment with EGTA and Subsequent Calcium Incubation

We demonstrated that mouse spermatozoa cleave their DNA into ~50 kb loop-sized fragments with topoisomerase IIB when treated with MnCl(2) and CaCl(2) in a process we term sperm chromatin fragmentation (SCF). SCF can be reversed by EDTA. A nuclease then further degrades the DNA in a process we term s...

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Bibliografiske detaljer
Main Authors: Boaz, Segal M., Dominguez, Kenneth, Shaman, Jeffrey A., Ward, W. Steven
Format: Artigo
Sprog:Inglês
Udgivet: 2008
Fag:
Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2679532/
https://ncbi.nlm.nih.gov/pubmed/17879959
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jcb.21549
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