Simple, efficient purification of filamentous hemagglutinin and pertussis toxin from Bordetella pertussis by hydrophobic and affinity interaction.

Two major antigens of Bordetella pertussis, filamentous hemagglutinin (FHA) and pertussis toxin (PT), were efficiently purified from culture filtrate by exploiting their relative hydrophobicities and differences in affinity to sialic acid-containing protein. High yields of FHA (40 to 80 mg/liter) an...

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Veröffentlicht in:	J Clin Microbiol
Hauptverfasser:	Skelton, S K, Wong, K H
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1990
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC267867/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2351723/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.28.5.1062-1065.1990
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