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Microculture assay for isolation of human immunodeficiency virus type 1 and for titration of infected peripheral blood mononuclear cells.

To define the optimal conditions for human immunodeficiency virus (HIV) detection in microcultures, experiments were conducted with different ratios of patient and donor peripheral blood mononuclear cells (PBMCs). Donor/patient PBMC ratios ranged from 1:1 to 1:125. Optimal results were obtained when...

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Udgivet i:J Clin Microbiol
Principais autores: Dimitrov, D H, Melnick, J L, Hollinger, F B
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1990
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC267785/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2332470/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.28.4.734-737.1990
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