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Method for improved extraction of DNA from Nocardia asteroides.

In a variation of standard DNA extraction methods, Nocardia asteroides was repeatedly exposed to sodium dodecyl sulfate at 60 degrees C for 30 min; each extraction was followed by centrifugation, removal of the nucleic acid-rich supernatant, and suspension of the cell pellet in fresh sodium dodecyl...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Principais autores: Loeffelholz, M J, Scholl, D R
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1989
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC267689/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2671036/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.27.8.1880-1881.1989
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