Quantitative determination of staphylococcal enterotoxin A by an enzyme-linked immunosorbent assay using a combination of polyclonal and monoclonal antibodies and biotin-streptavidin interaction.

A sandwich enzyme-linked immunosorbent assay to detect staphylococcal enterotoxin A (SEA) was developed by using monoclonal antibodies (MAb) to SEA as primary capture antibodies. The antigen was detected with purified rabbit anti-SEA antibody as the secondary antibody. The secondary antibody was ide...

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Bibliographic Details
Published in:J Clin Microbiol
Main Author: Edwin, C
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1989
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC267602/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2768439/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.27.7.1496-1501.1989
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