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Comparison of colorimetric, fluorescent, and enzymatic amplification substrate systems in an enzyme immunoassay for detection of DNA-RNA hybrids.

The monoclonal antibody solution hybridization assay is a novel enzyme immunoassay for detection of RNA with a biotinylated DNA probe. To increase the sensitivity of this test, a fluorescent substrate and an enzymatic amplification cycling system were compared with a conventional colorigenic substra...

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Bibliografiske detaljer
Main Authors: Coutlee, F, Viscidi, R P, Yolken, R H
Format: Artigo
Sprog:Inglês
Udgivet: 1989
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC267471/
https://ncbi.nlm.nih.gov/pubmed/2473088
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