A simple and high-sensitivity method for analysis of ubiquitination and polyubiquitination based on wheat cell-free protein synthesis

BACKGROUND: Ubiquitination is mediated by the sequential action of at least three enzymes: the E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme) and E3 (ubiquitin ligase) proteins. Polyubiquitination of target proteins is also implicated in several critical cellular processes. Alth...

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Main Authors: Takahashi, Hirotaka, Nozawa, Akira, Seki, Motoaki, Shinozaki, Kazuo, Endo, Yaeta, Sawasaki, Tatsuya
Formato: Artigo
Idioma:Inglês
Publicado: BioMed Central 2009
Assuntos:
Methodology Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2674041/
https://ncbi.nlm.nih.gov/pubmed/19348673
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2229-9-39
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