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Highly sensitive and quantitative FRET-FLIM imaging in single dendritic spines using improved non-radiative YFP
Two-photon fluorescence lifetime imaging microscopy (TPFLIM) enables the quantitative measurements of fluorescence resonance energy transfer (FRET) in small subcellular compartments in light scattering tissue. We evaluated and optimized the FRET pair of mEGFP (monomeric EGFP with the A206K mutation)...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2673728/ https://ncbi.nlm.nih.gov/pubmed/18512154 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s11068-008-9024-9 |
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