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Early serodiagnosis of acute human cytomegalovirus infection by enzyme-linked immunosorbent assay using recombinant antigens.
DNA fragments from eight different reading frames of human cytomegalovirus (HCMV) were generated by PCR and subsequently cloned and expressed in Escherichia coli in fusion with glutathione S-transferase. The recombinant viral antigens were evaluated in immunoblot analyses. The most reactive antigens...
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| Main Authors: | , , , , , , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1994
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC267166/ https://ncbi.nlm.nih.gov/pubmed/8027354 |
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