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Preparation of heteroduplex EGFP plasmid for in vivo mismatch repair activity assay
Preparation of heteroduplexes in large quantities with high purity is essential for the measurement of DNA mismatch repair (MMR) activity. Here we report a rapid, less labor-intensive method for the preparation of a heteroduplex plasmid that expresses the enhanced green fluorescent protein (EGFP) if...
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| Auteurs principaux: | , , , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2009
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2670966/ https://ncbi.nlm.nih.gov/pubmed/19248754 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2009.02.020 |
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