Enzyme-linked immunosorbent fluorescence assay and high-pressure liquid chromatography for analysis of humoral immune responses to Coxiella burnetti proteins.

A microtiter enzyme-linked immunosorbent fluorescence assay based on alkaline phosphatase conjugate and 4-methylumbelliferyl phosphate as fluorogenic substrate was developed and adapted to quantitatively analyze immunoglobulin G subclass 1 (IgG1) and IgG2 responses of vaccinated and infected cattle...

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Main Authors: Schmeer, N, Müller, H P, Baumgärtner, W, Wieda, J, Krauss, H
Format: Artigo
Jezik:Inglês
Izdano: 1988
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC266937/
https://ncbi.nlm.nih.gov/pubmed/3068247
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