High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains—selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated (15)N-labeled phCG

The methylotrophic yeast Pichia pastoris is widely used for the production of recombinant glycoproteins. With the aim to generate biologically active (15)N-labeled glycohormones for conformational studies focused on the unravelling of the NMR structures in solution, the P. pastoris strains GS115 and...

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Main Authors: Blanchard, Véronique, Gadkari, Rupali A., George, Albert V. E., Roy, Satarupa, Gerwig, Gerrit J., Leeflang, Bas R., Dighe, Rajan R., Boelens, Rolf, Kamerling, Johannis P.
Formato: Artigo
Idioma:Inglês
Publicado em: Springer US 2008
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2668595/
https://ncbi.nlm.nih.gov/pubmed/18274893
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10719-007-9082-8
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