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Accurate Real-Time PCR Strategy for Monitoring Bloodstream Parasitic Loads in Chagas Disease Patients

BACKGROUND: This report describes a real-time PCR (Q-PCR) strategy to quantify Trypanosoma cruzi (T. cruzi) DNA in peripheral blood samples from Chagas disease patients targeted to conserved motifs within the repetitive satellite sequence. METHODOLOGY/PRINCIPAL FINDINGS: The Q-PCR has a detection li...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Duffy, Tomas, Bisio, Margarita, Altcheh, Jaime, Burgos, Juan Miguel, Diez, Mirta, Levin, Mariano Jorge, Favaloro, Roberto Rene, Freilij, Hector, Schijman, Alejandro Gabriel
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2009
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2667272/
https://ncbi.nlm.nih.gov/pubmed/19381287
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pntd.0000419
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