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Structural and Thermodynamic Characterization of T4 Lysozyme Mutants and the Contribution of Internal Cavities to Pressure Denaturation

Using small angle X-ray scattering (SAXS) and tryptophan fluorescence spectroscopy, we have identified multiple compact denatured states of a series of T4 lysozyme mutants that are stabilized by high pressures. Recent studies imply that the mechanism of pressure denaturation is the penetration of wa...

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Bibliografiska uppgifter
Huvudupphovsmän: Ando, Nozomi, Barstow, Buz, Baase, Walter A., Fields, Andrew, Matthews, Brian W., Gruner, Sol M.
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2008
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Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC2664309/
https://ncbi.nlm.nih.gov/pubmed/18816066
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi801287m
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