Integral and peripheral association of proteins and protein complexes with Yersinia pestis inner and outer membranes

Yersinia pestis proteins were sequentially extracted from crude membranes with a high salt buffer (2.5 M NaBr), an alkaline solution (180 mM Na(2)CO(3), pH 11.3) and membrane denaturants (8 M urea, 2 M thiourea and 1% amidosulfobetaine-14). Separation of proteins by 2D gel electrophoresis was follow...

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Detalhes bibliográficos
Main Authors: Pieper, Rembert, Huang, Shih-Ting, Clark, David J, Robinson, Jeffrey M, Alami, Hamid, Parmar, Prashanth P, Suh, Moo-Jin, Kuntumalla, Srilatha, Bunai, Christine L, Perry, Robert D, Fleischmann, Robert D, Peterson, Scott N
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2009
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2663777/
https://ncbi.nlm.nih.gov/pubmed/19228400
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1477-5956-7-5
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