Development of a rapid and sensitive polymerase chain reaction assay for detection of bovine herpesvirus type 1 in bovine semen.

We developed a polymerase chain reaction (PCR) assay to detect bovine herpesvirus type 1 (BHV-1) in bovine semen. Since bovine semen contains components that inhibit PCR amplification, a protocol was developed to purify BHV-1 DNA from bovine semen. To identify failures of PCR amplification, we used...

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Main Authors: van Engelenburg, F A, Maes, R K, van Oirschot, J T, Rijsewijk, F A
Formato: Artigo
Idioma:Inglês
Publicado: 1993
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Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC266363/
https://ncbi.nlm.nih.gov/pubmed/8308103
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