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Direct detection of Escherichia coli Shiga-like toxin genes in primary fecal cultures by polymerase chain reaction.

A single pair of oligonucleotide primers was used for polymerase chain reaction amplification of a 212- or 215-bp region of Escherichia coli Shiga-like toxin (SLT) genes from crude fecal culture extracts. Genes were typed by hybridization of the polymerase chain reaction products to SLT-I- or SLT-II...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Principais autores: Paton, A W, Paton, J C, Goldwater, P N, Manning, P A
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1993
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC266220/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8263203/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.31.11.3063-3067.1993
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