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Expression of A and B subunits of Shiga-like toxin II as fusions with glutathione S-transferase and their potential for use in seroepidemiology.

We used the plasmid vector pGEX-2T for the expression of recombinant subunits of Shiga-like toxin II (SLT-II). The 5' terminus of the genes that code for either the SLT-IIA or SLT-IIB subunits was genetically fused to the 3' terminus of the gene coding for the enzyme glutathione S-transferase, which...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Principais autores: Gunzer, F, Karch, H
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1993
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC265944/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8253955/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.31.10.2604-2610.1993
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