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Directed evolution of homing endonuclease I-SceI with altered sequence specificity
Homing endonucleases recognize specific long DNA sequences and catalyze double-stranded breaks that significantly stimulate homologous recombination, representing an attractive tool for genome targeting and editing. We previously described a two-plasmid selection system that couples enzymatic DNA cl...
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| Autori principali: | , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
2009
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2659007/ https://ncbi.nlm.nih.gov/pubmed/19176595 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzp001 |
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