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Physicochemical characterization of porcine pararotavirus and detection of virus and viral antibodies using cell culture immunofluorescence.
A cell culture immunofluorescence (CCIF) assay was optimized for detection of porcine pararotavirus (group C rotavirus) in intestinal contents. The greatest viral infectivity was observed when MA104 cells (5 days after subculturing) were rinsed and refed in serum-free medium before inoculation, panc...
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| Main Authors: | , , , |
|---|---|
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
1987
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC265881/ https://ncbi.nlm.nih.gov/pubmed/3029161 |
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