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Gene probes for identification of the botulinal neurotoxin gene and specific identification of neurotoxin types B, E, and F.

A polymerase chain reaction method was developed for the specific detection of the botulinum neurotoxin (BoNT) gene of Clostridium botulinum. Degenerate oligonucleotide primers, designed from the nucleotide sequence of the heavy chain of the BoNT gene, amplified a specific fragment of approximately...

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Bibliografiske detaljer
Udgivet i:J Clin Microbiol
Principais autores: Campbell, K D, Collins, M D, East, A K
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1993
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC265744/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8408542/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.31.9.2255-2262.1993
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