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Proliferation of rat Pneumocystis carinii on cells sheeted on microcarrier beads in spinner flasks.

A method of growing rat Pneumocystis carinii with human embryonic lung fibroblasts (HEL-299 cells) sheeted onto microcarrier beads has been developed. This method allows production of large quantities of P. carinii organisms with very little contamination of host cells. A fivefold increase in the nu...

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Published in:J Clin Microbiol
Main Authors: Lee, C H, Bauer, N L, Shaw, M M, Durkin, M M, Bartlett, M S, Queener, S F, Smith, J W
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1993
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Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC265602/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8315014/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.31.6.1659-1662.1993
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