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Detection of aquareovirus RNA in fish tissues by nucleic acid hybridization with a cloned cDNA probe.

A nucleic acid hybridization assay was developed to rapidly detect small quantities of aquareovirus RNAs in infected cells and organs. Cloned cDNA copies were synthesized from the genomic RNA of the SBR strain of aquareovirus. By using cloned cDNA probes, aquareovirus RNAs were detected in spleen an...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Principais autores: Subramanian, K, Lupiani, B, Hetrick, F M, Samal, S K
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1993
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC265588/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8391024/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.31.6.1612-1614.1993
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