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Rapid Oligonucleotide-Based Recombineering of the Chromosome of Salmonella enterica

Recombinant engineering using Red recombinase-based approaches offers efficient and rapid approaches to deletion and modification of genes. Here we describe a novel application of Red recombinant engineering that enables direct manipulation of chromosomal loci by electroporation with short synthetic...

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Detalhes bibliográficos
Main Authors: Gerlach, Roman G., Jäckel, Daniela, Hölzer, Stefanie U., Hensel, Michael
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2655478/
https://ncbi.nlm.nih.gov/pubmed/19151186
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02509-08
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