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Super-resolution imaging in live Caulobacter crescentus cells using photoswitchable EYFP
The commonly used, monomeric EYFP enabled imaging of intracellular protein structures beyond the optical resolution limit (‘super-resolution’ imaging) in living cells. By combining photoinduced activation of single EYFP fusions and time-lapse imaging, we obtained sub–40 nm resolution images of the f...
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| Autors principals: | , , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2008
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2655310/ https://ncbi.nlm.nih.gov/pubmed/18794860 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/NMETH.1258 |
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