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Purification of prenylated proteins by affinity chromatography on cyclodextrin-modified agarose

Although protein prenylation is widely studied, there are few good methods for isolating prenylated proteins from their non-prenylated relatives. We report that crosslinked agarose (e.g., Sepharose) chromatography media that has been chemically functionalized with β-cyclodextrin (β-CD) is extremely...

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Detalhes bibliográficos
Main Authors: Chung, Jinhwa A., Wollack, James W., Hovlid, Marisa L., Okesli, Ayse, Chen, Yan, Mueller, Joachim D., Distefano, Mark D., Taton, T. Andrew
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2654400/
https://ncbi.nlm.nih.gov/pubmed/18834849
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2008.09.007
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