Use of nonradioactive DNA hybridization for identification of enterotoxigenic Escherichia coli harboring genes for colonization factor antigen I, coli surface antigen 4, or putative colonization factor O166.

We developed an accurate nonradioactive colony hybridization assay (NCHA) using a digoxigenin-labeled polynucleotide probe and an antidigoxigenin alkaline phosphatase conjugate for the identification of enterotoxigenic Escherichia coli (ETEC) harboring genes for colonization factor antigen I (CFA/I)...

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Bibliografiske detaljer
Udgivet i:J Clin Microbiol
Main Authors: Sommerfelt, H, Grewal, H M, Gaastra, W, Svennerholm, A M, Bhan, M K
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1992
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC265388/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1629340/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.30.7.1823-1828.1992
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