Polymerase chain reaction for diagnosis of enterohemorrhagic Escherichia coli infection and hemolytic-uremic syndrome.

Two pairs of oligonucleotide primers were designed to amplify fragments of the genes for Shiga-like toxin I (SLT-I) and SLT-II in a single reaction. A 370-bp segment and a 283-bp segment were amplified for SLT-I and SLT-II, respectively. The specificities of the polymerase chain reaction (PCR) ampli...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Main Authors: Brian, M J, Frosolono, M, Murray, B E, Miranda, A, Lopez, E L, Gomez, H F, Cleary, T G
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1992
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC265384/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1629337/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.30.7.1801-1806.1992
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