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Using multiplexed regulation of luciferase activity and GFP translocation to screen for FOXO modulators

BACKGROUND: Independent luciferase reporter assays and fluorescent translocation assays have been successfully used in drug discovery for several molecular targets. We developed U2transLUC, an assay system in which luciferase and fluorescent read-outs can be multiplexed to provide a powerful cell-ba...

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Autors principals: Zanella, Fabian, Rosado, Aranzazú, Garcia, Beatriz, Carnero, Amancio, Link, Wolfgang
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2009
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2651847/
https://ncbi.nlm.nih.gov/pubmed/19243599
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2121-10-14
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