Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction.

We report on the development and application of a rapid assay for detecting and typing dengue viruses. Oligonucleotide consensus primers were designed to anneal to any of the four dengue virus types and amplify a 511-bp product in a reverse transcriptase-polymerase chain reaction (PCR). First, we pr...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Main Authors: Lanciotti, R S, Calisher, C H, Gubler, D J, Chang, G J, Vorndam, A V
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1992
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC265106/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1372617/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.30.3.545-551.1992
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