Optimization and standardization of an enzyme-linked immunosorbent assay protocol for serodiagnosis of Actinobacillus pleuropneumoniae serotype 5.

An indirect enzyme-linked immunosorbent assay protocol has been optimized with special emphasis given to assay standardization and quality control. Technical aspects such as choice of a microplate, antigen immobilization, buffer composition, optimal screening dilution of sera, and kinetics of the en...

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Pubblicato in:	J Clin Microbiol
Autori principali:	Trottier, Y L, Wright, P F, Larivière, S
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1992
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC264994/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1734068/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.30.1.46-53.1992
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