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Identification of Protein-Protein Interactions and Topologies in Living Cells with Chemical Cross-linking and Mass Spectrometry

We present results from a novel strategy that enables concurrent identification of protein-protein interactions and topologies in living cells without specific antibodies or genetic manipulations for immuno-/affinity purifications. The strategy consists of (i) a chemical cross-linking reaction: inta...

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Detalhes bibliográficos
Main Authors: Zhang, Haizhen, Tang, Xiaoting, Munske, Gerhard R., Tolic, Nikola, Anderson, Gordon A., Bruce, James E.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2649805/
https://ncbi.nlm.nih.gov/pubmed/18936057
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M800232-MCP200
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