A Toolkit and Robust Pipeline for the Generation of Fosmid-Based Reporter Genes in C. elegans

Engineering fluorescent proteins into large genomic clones, contained within BACs or fosmid vectors, is a tool to visualize and study spatiotemporal gene expression patterns in transgenic animals. Because these reporters cover large genomic regions, they most likely capture all cis-regulatory inform...

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Bibliographic Details
Main Authors: Tursun, Baris, Cochella, Luisa, Carrera, Inés, Hobert, Oliver
Format: Artigo
Language:Inglês
Published: Public Library of Science 2009
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2649505/
https://ncbi.nlm.nih.gov/pubmed/19259264
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0004625
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