In vitro cloning of complex mixtures of DNA on microbeads: Physical separation of differentially expressed cDNAs

We describe a method for cloning nucleic acid molecules onto the surfaces of 5-μm microbeads rather than in biological hosts. A unique tag sequence is attached to each molecule, and the tagged library is amplified. Unique tagging of the molecules is achieved by sampling a small fraction (1%) of a ve...

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Autori principali: Brenner, Sydney, Williams, Steven R., Vermaas, Eric H., Storck, Thorsten, Moon, Keith, McCollum, Christie, Mao, Jen-I, Luo, Shujun, Kirchner, James J., Eletr, Sam, DuBridge, Robert B., Burcham, Timothy, Albrecht, Glenn
Natura: Artigo
Lingua:Inglês
Pubblicazione: The National Academy of Sciences 2000
Soggetti:
Biological Sciences
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC26493/
https://ncbi.nlm.nih.gov/pubmed/10677516
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