Phleomycin Increases Transformation Efficiency and Promotes Single Integrations in Schizophyllum commune

Phleomycin is mutagenic by introducing double-strand breaks in DNA. The ble gene of Streptoalloteychus hindustanus, which confers resistance to this substance, is widely used as a selection marker for transformation. Schizophyllum commune grows on 25 μg of phleomycin ml(−1) after introduction of a r...

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Bibliografiske detaljer
Main Authors: van Peer, Arend F., de Bekker, Charissa, Vinck, Arman, Wösten, Han A. B., Lugones, Luis G.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2009
Fag:
Mycology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2648172/
https://ncbi.nlm.nih.gov/pubmed/19114524
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02162-08
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