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Sensitive isothermal detection of nucleic-acid sequence by primer generation–rolling circle amplification
A simple isothermal nucleic-acid amplification reaction, primer generation–rolling circle amplification (PG–RCA), was developed to detect specific nucleic-acid sequences of sample DNA. This amplification method is achievable at a constant temperature (e.g. 60°C) simply by mixing circular single-stra...
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| Main Authors: | , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
2009
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2647323/ https://ncbi.nlm.nih.gov/pubmed/19106144 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn1014 |
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