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Sensitive isothermal detection of nucleic-acid sequence by primer generation–rolling circle amplification

A simple isothermal nucleic-acid amplification reaction, primer generation–rolling circle amplification (PG–RCA), was developed to detect specific nucleic-acid sequences of sample DNA. This amplification method is achievable at a constant temperature (e.g. 60°C) simply by mixing circular single-stra...

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Hlavní autoři: Murakami, Taku, Sumaoka, Jun, Komiyama, Makoto
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2009
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2647323/
https://ncbi.nlm.nih.gov/pubmed/19106144
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn1014
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