Simple high-cell density fed-batch technique for high-level recombinant protein production with Pichia pastoris: Application to intracellular production of Hepatitis B surface antigen

BACKGROUND: Hepatitis B is a serious global public health concern. Though a safe and efficacious recombinant vaccine is available, its use in several resource-poor countries is limited by cost. We have investigated the production of Hepatitis B virus surface antigen (HBsAg) using the yeast Pichia pa...

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Main Authors: Gurramkonda, Chandrasekhar, Adnan, Ahmad, Gäbel, Thomas, Lünsdorf, Heinrich, Ross, Anton, Nemani, Satish Kumar, Swaminathan, Sathyamangalam, Khanna, Navin, Rinas, Ursula
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2009
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2646686/
https://ncbi.nlm.nih.gov/pubmed/19208244
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-8-13
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