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Crystallographic, Spectroscopic, and Computational Analysis of a Flavin-C4a-Oxygen Adduct in Choline Oxidase

Flavin C4a-OOH and C4a-OH adducts are critical intermediates proposed in many flavoenzyme reaction mechanisms, but they are rarely detected even by rapid transient kinetics methods. We observe a trapped flavin C4a-OH or C4a-OO(H) adduct by single crystal spectroscopic methods and in the 1.86 Å resol...

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Detalhes bibliográficos
Main Authors: Orville, Allen M., Lountos, George T., Finnegan, Steffan, Gadda, Giovanni, Prabhakar, Rajeev
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2646362/
https://ncbi.nlm.nih.gov/pubmed/19133805
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi801918u
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