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Using NMR to Distinguish Viscosity Effects from Non-specific Protein Binding under Crowded Conditions

[Image: see text] Conventional NMR approaches to detect weak protein binding and aggregation are hindered by the increased viscosity brought about by crowding. We describe a simple and reliable NMR method to distinguish viscosity effects from binding and aggregation under crowded conditions.

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Bibliographische Detailangaben
Hauptverfasser: Li, Conggang, Pielak, Gary J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2009
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2645536/
https://ncbi.nlm.nih.gov/pubmed/19140727
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja808428d
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