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Using NMR to Distinguish Viscosity Effects from Non-specific Protein Binding under Crowded Conditions
[Image: see text] Conventional NMR approaches to detect weak protein binding and aggregation are hindered by the increased viscosity brought about by crowding. We describe a simple and reliable NMR method to distinguish viscosity effects from binding and aggregation under crowded conditions.
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2645536/ https://ncbi.nlm.nih.gov/pubmed/19140727 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja808428d |
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