Cloning and expression in Escherichia coli of the streptolysin O determinant from Streptococcus pyogenes: characterization of the cloned streptolysin O determinant and demonstration of the absence of substantial homology with determinants of other thiol-activated toxins.

A gene bank of Streptococcus pyogenes Richards was constructed in Escherichia coli by using the bacteriophage replacement vector lambda L47.1, and hybrid phage expressing streptolysin O (SLO) were identified among the recombinants. DNA sequences encoding SLO were subcloned from an slo+ hybrid phage...

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Bibliographic Details
Published in:Infect Immun
Main Authors: Kehoe, M, Timmis, K N
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1984
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC264252/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6321351/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.43.3.804-810.1984
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