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Reverse transcription-PCR detection of LaCrosse virus in mosquitoes and comparison with enzyme immunoassay and virus isolation.

A reverse transcription-PCR (RT-PCR) assay was developed and compared with enzyme immunoassay (EIA) and virus isolation for detecting LaCrosse virus (LAC) in mosquito pools. All three techniques were able to detect a single LAC-infected mosquito in a pool of 99 negative mosquitoes. Virus isolation w...

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Bibliografiske detaljer
Udgivet i:J Clin Microbiol
Principais autores: Wasieloski, L P, Rayms-Keller, A, Curtis, L A, Blair, C D, Beaty, B J
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1994
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC263945/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7814527/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.32.9.2076-2080.1994
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