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Identification of fission yeast nuclear markers using random polypeptide fusions with green fluorescent protein

We describe a method for identifying genes encoding proteins with stereospecific intracellular localizations in the fission yeast Schizosaccharomyces pombe. Yeast are transformed with a gene library in which S. pombe genomic sequences are fused to the gene encoding the Aequorea victoria green fluore...

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Autori principali: Sawin, Kenneth E., Nurse, Paul
Natura: Artigo
Lingua:Inglês
Pubblicazione: The National Academy of Sciences of the USA 1996
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC26371/
https://ncbi.nlm.nih.gov/pubmed/8986778
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