Crystallization and preliminary X-ray analysis of 2,3-diketo-5-methylthiopentyl-1-phosphate enolase from Bacillus subtilis

2,3-Diketo-5-methylthiopentyl-1-phosphate enolase (DK-MTP-1P enolase) from Bacillus subtilis was crystallized using the hanging-drop vapour-diffusion method. Crystals grew using PEG 3350 as the precipitant at 293 K. The crystals diffracted to 2.3 Å resolution at 100 K using synchrotron radiation and...

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Detalhes bibliográficos
Main Authors: Tamura, Haruka, Ashida, Hiroki, Koga, Shogo, Saito, Yohtaro, Yadani, Tomonori, Kai, Yasushi, Inoue, Tsuyoshi, Yokota, Akiho, Matsumura, Hiroyoshi
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2009
Assuntos:
Crystallization Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2635871/
https://ncbi.nlm.nih.gov/pubmed/19194007
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430910804311X
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