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Expression, purification, crystallization and preliminary X-ray diffraction analysis of the soluble domain of PPA0092, a putative nitrite reductase from Propionibacterium acnes

The soluble domain (residues 483–913) of PPA0092, a putative copper-containing nitrite reductase from Propionibacterium acnes KPA171202, has been overexpressed in Escherichia coli. The purified recombinant protein was crystallized using the hanging-drop vapour-diffusion method. X-ray diffraction dat...

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書目詳細資料
Main Authors: Nojiri, Masaki, Shirota, Felicia, Hira, Daisuke, Suzuki, Shinnichiro
格式: Artigo
語言:Inglês
出版: International Union of Crystallography 2009
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2635855/
https://ncbi.nlm.nih.gov/pubmed/19194001
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309108040207
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