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Detection of hepatitis C virus RNA by a combined reverse transcription-polymerase chain reaction assay.

Amplification of RNA by the polymerase chain reaction (PCR) is normally a two-step process requiring separate enzymes and buffer conditions. We describe a combined reverse transcription-PCR (RT-PCR) assay for hepatitis C virus (HCV) RNA amplification in which a single enzyme and buffer condition are...

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Bibliographic Details
Published in:J Clin Microbiol
Main Authors: Young, K K, Resnick, R M, Myers, T W
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1993
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC263581/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8385151/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.31.4.882-886.1993
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