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Upscaled CTAB-Based DNA Extraction and Real-Time PCR Assays for Fusarium culmorum and F. graminearum DNA in Plant Material with Reduced Sampling Error

Fusarium graminearum Schwabe (Gibberella zeae Schwein. Petch.) and F. culmorum W.G. Smith are major mycotoxin producers in small-grain cereals afflicted with Fusarium head blight (FHB). Real-time PCR (qPCR) is the method of choice for species-specific, quantitative estimation of fungal biomass in pl...

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Bibliografiske detaljer
Main Authors: Brandfass, Christoph, Karlovsky, Petr
Format: Artigo
Sprog:Inglês
Udgivet: Molecular Diversity Preservation International (MDPI) 2008
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2635625/
https://ncbi.nlm.nih.gov/pubmed/19330077
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms9112306
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