Isolation of cDNA encoding the catalytic site of phosphatidylinositol-specific phospholipase C from Coffea arabica L.: Recombinant expression and peptide purification

A cDNA encoding the catalytic site of a phosphatidylinositol-specific phospholipase C (PI-PLC) was isolated from Coffea arabica suspension cells. The cDNA (designated CaPLC) encodes a polypeptide of 308 amino acids, containing the catalytic X and Y domains, and has 99% identity to the soybean gene....

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Detalhes bibliográficos
Main Authors: Sánchez-Cach, Lucila A, Ortiz-García, Matilde M, Minero-García, Yereni, Muñoz-Sánchez, J Armando, Hernández-Sotomayor, SM Teresa, Suárez-Solís, Víctor M, De los Santos-Briones, César
Formato: Artigo
Idioma:Inglês
Publicado em: Landes Bioscience 2008
Assuntos:
Short Communication
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2633734/
https://ncbi.nlm.nih.gov/pubmed/19513191
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