Detection of North American West Nile virus in animal tissue by a reverse transcription-nested polymerase chain reaction assay.

A traditional single-stage reverse transcription-polymerase chain reaction (RT-PCR) procedure is effective in determining West Nile (WN) virus in avian tissue and infected cell cultures. However, the procedure lacks the sensitivity to detect WN virus in equine tissue. We describe an RT-nested PCR (R...

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Bibliografiske detaljer
Main Authors: Johnson, D J, Ostlund, E N, Pedersen, D D, Schmitt, B J
Format: Artigo
Sprog:Inglês
Udgivet: Centers for Disease Control and Prevention 2001
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2631755/
https://ncbi.nlm.nih.gov/pubmed/11585541
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