Direct demonstration of the presence of coordinated sulfate in the reaction pathway of Arabidopsis thaliana sulfite oxidase using (33)S labeling and ESEEM spectroscopy

Sulfite oxidase from Arabidopsis thaliana has been reduced at pH = 6 with sulfite labeled with (33)S (nuclear spin I = 3/2), followed by reoxidation by ferricyanide to generate the Mo(V) state of the active center. To obtain information about the hyperfine interaction (hfi) of (33)S with Mo(V), cont...

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Hlavní autoři: Astashkin, Andrei V., Johnson-Winters, Kayunta, Klein, Eric L., Byrne, Robert S., Hille, Russ, Raitsimring, Arnold M., Enemark, John H.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2007
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2630056/
https://ncbi.nlm.nih.gov/pubmed/17983221
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja0704885
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